Rubisco in spinach leaves

Get Access Chromatography Of Photosynthetic Pigments In Spinach Biology Essay The purpose of this experiment was to separate and insulate the different photosynthetic pigments, found on Spinacia oleracea foliages and to pull out them utilizing the paper chromatography method.

Rubisco in spinach leaves

Click on the following links for more information. This tree includes only several representative species. Cellular role predicted cellular role Literature relevant references for this domain Primary literature is listed below; Automatically-derived, secondary literature is also avaliable. Rbcs-2A and Rbcs-3A differ from each other by Thus the tobacco gene seems to be phylogenetically asclosely related to the tomato genes Rbcs-2A and Rbcs-3A as the latter twoare to each other, and more closely related to them than Rbcs-1 is.

However, the mature part of the polypeptide encoded by the tobacco RBCSgene differs by five and six amino acids from the corresponding region inthe polypeptides encoded by Rbcs-2A and Rbcs-3A, respectively, while thesetwo tomato RBCS polypeptides differ from each other in the mature part bya single amino acid.

Rbcs-1, whose nucleotide sequence shows higherdivergence from both the tobacco RBCS gene and Rbcs-2A and Rbcs-3A,encodes a polypeptide whose mature part differs by eight amino acids fromthe corresponding region in the tobacco polypeptide but only by three andfour amino acids from the corresponding regions of Rbcs-2A- andRbcs-3A-encoded polypeptides, respectively.

Thus, it appears that in thetomato selection has maintained near uniformity of the coding informationin the portion of the RBCS genes encoding the mature polypeptides. The genes encoding the small subunit of ribulose-1,5-bisphosphatecarboxylase are expressed differentially in petunia leaves.

Of the five rbcS genes, two ssu11A and ssu8 are expressed at high levels in petunia leaves. Structuralcomparisons of ssu8 and ssu11A revealed that the coding sequence of ssu8is interrupted by three introns, while the coding sequence of ssu11A isinterrupted by two introns.

The positions of the first two introns areidentical, the third intron in ssu8 is located in a highly conservedregion of the protein.

Rubisco in spinach leaves

The 5' and 3' flanking sequences of ssu11A arehighly homologous to the 5' and 3' flanking sequences of ssu8. S1 nucleasemapping was used to locate the start of transcription of ssu8 and ssu11Aand showed that ssu8 mRNA leader differs in sequence from the ssu11A mRNAleader.Partially purified Rubisco from spinach was purchased from Sigma and purified by gel chromatography with Sephacryl S (97· cm, Pharmacia) in 50 mM Tris-HCl, pH 8 and 50 mM NaCl at 4 °C.

To this end, researchers from Uppsala University are using neutrons at the Department of Energy’s (DOE’s) Oak Ridge National Laboratory to determine the protein structure of a RuBisCO sample obtained from spinach leaves.

RuBisCO. Ribulose bisphosphate carboxylase/oxygenase (RUBISCO) is a kDa enzyme, the most abundant protein on earth, that carries out the photosynthetic fixation of carbon dioxide in the chloroplast.

Isolation and Identification of Rubisco Extracted from To isolate rubisco from spinach leaves, all of these techniques were employed.

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The known properties of rubisco were then compared to the properties of the unknown proteins extracted from the spinach . Plants, algae, and other organisms produce the RuBisCO enzyme to convert carbon dioxide from the atmosphere into energy-rich molecules, like glucose, that form carbohydrates and other organic.

Spinach used in neutron studies could unearth secret to stronger plant growth; Spinach used in neutron studies could unearth secret to stronger plant growth.

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